Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH). Because the embryos can be distinguished by genotype after ISH. comparing gene expression between wild-type and mutant siblings can be done blinded and in parallel. https://www.roneverhart.com/Turbosound-TS-18SW700-8A-18-Low-Frequency-Loudspeaker-p31480/